5HVJ

Crystal structure of LIMK1 D460N mutant in complex with AMP-PNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-24
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	54.987, 59.573, 98.276
Unit cell angles	90.00, 101.28, 90.00

Refinement procedure

Resolution	39.979 - 2.200
R-factor	0.208
Rwork	0.206
R-free	0.25220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3s95
RMSD bond length	0.002
RMSD bond angle	0.641
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (dev_1819)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.979	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.156
Number of reflections	31929
<I/σ(I)>	12.8	1.1
Completeness [%]	100.0	100
Redundancy	14.3	13.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	18-24% PEG 3350, 0.2 M potassium citrate