5HUA
Structure of C. glabrata FKBP12-FK506 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-05-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 63 |
Unit cell lengths | 77.823, 77.823, 53.605 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 18.692 - 1.300 |
R-factor | 0.1538 |
Rwork | 0.153 |
R-free | 0.16400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.025 |
RMSD bond angle | 2.248 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 46.000 |
High resolution limit [Å] | 1.300 |
Number of reflections | 16318 |
<I/σ(I)> | 5.6 |
Completeness [%] | 87.0 |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG 3000, 0.1 M NaCl |