5HTO

Crystal structure of Plasmodium Vivax LDH in complex with a DNA aptamer called pL1 (tetrameric LDH in an asymmetric unit)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-02
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9796
Spacegroup name	P 21 21 21
Unit cell lengths	85.512, 86.539, 209.128
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.999 - 1.900
R-factor	0.1706
Rwork	0.169
R-free	0.20010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2a92
RMSD bond length	0.009
RMSD bond angle	1.110
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	25.000	25.000	1.930
High resolution limit [Å]	1.900	5.140	1.900
Rmerge	0.083	0.042	0.288
Number of reflections	122668
<I/σ(I)>	16.2
Completeness [%]	100.0	99.6	100
Redundancy	14.6	13.8	14.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	PEG-20K, magnesium ion, HEPES, Tirs, PEG-400