5HTK
Human Heart 6-Phosphofructo-2-Kinase/Fructose-2,6-Bisphosphatase (PFKFB2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CAMD BEAMLINE GCPCC |
Synchrotron site | CAMD |
Beamline | GCPCC |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2015-02-02 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.3808 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 106.523, 113.946, 133.154 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.550 - 2.010 |
R-factor | 0.1506 |
Rwork | 0.149 |
R-free | 0.17490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1k6m |
RMSD bond length | 0.016 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | HKL-2000 |
Refinement software | PHENIX ((dev_2000:)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 38.550 |
High resolution limit [Å] | 2.010 |
Number of reflections | 106633 |
<I/σ(I)> | 16.2 |
Completeness [%] | 98.0 |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 285 | 100mM MES, 0.5-3.0% polyethylene glycol 8000, 13-16% polyethylene glycol 3350, and 3% dioxane |