5HT6
Crystal structure of the SET domain of the human MLL5 methyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-18 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.07245 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 65.900, 65.900, 112.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.933 - 2.093 |
| R-factor | 0.2116 |
| Rwork | 0.209 |
| R-free | 0.24240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.841 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.21) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.174 | 2.210 |
| High resolution limit [Å] | 2.093 | 2.100 |
| Rmerge | 0.390 | |
| Number of reflections | 17289 | |
| <I/σ(I)> | 22 | 2 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 8 | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M NH4Cl, 12 % (w/v) PEG 3350 |
