5HRT

Crystal structure of mouse autotaxin in complex with a DNA aptamer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-11
Detector	RAYONIX MX225HE
Wavelength(s)	1.0
Spacegroup name	C 2 2 21
Unit cell lengths	120.062, 208.757, 90.036
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.152 - 1.997
R-factor	0.1647
Rwork	0.164
R-free	0.20180
RMSD bond length	0.018
RMSD bond angle	1.491
Phasing software	MOLREP
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	45.200
High resolution limit [Å]	1.997
Number of reflections	75910
<I/σ(I)>	15.5
Completeness [%]	98.8
Redundancy	14.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	PEG3350, calcium chloride, tris