5HRE
The crystal structure of AsfvPolX:DNA3 binary complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-01-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9793 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 64.144, 88.785, 43.349 |
Unit cell angles | 90.00, 94.61, 90.00 |
Refinement procedure
Resolution | 26.870 - 1.750 |
R-factor | 0.19709 |
Rwork | 0.195 |
R-free | 0.23594 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5hr9 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.255 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 1.750 |
Number of reflections | 22860 |
<I/σ(I)> | 26.8 |
Completeness [%] | 98.7 |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 20% PEG 3350, 0.2M Potassium Formate |