5HP3

Human Adenosine Deaminase Acting on dsRNA (ADAR2) bound to dsRNA sequence derived from S. cerevisiae BDF2 gene with AC mismatch at reaction site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-01-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	81.510, 107.210, 120.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.327 - 3.091
R-factor	0.1668
Rwork	0.163
R-free	0.23790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zy7
RMSD bond length	0.010
RMSD bond angle	1.365
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.140	3.170
High resolution limit [Å]	3.090	3.090
Rmerge	0.116	0.687
Number of reflections	19369
<I/σ(I)>	10.56	1.75
Completeness [%]	96.8	89.1
Redundancy	3.31	3.19
CC(1/2)	0.993

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	10% PEG3350, 15% glycerol, 0.1 M MES/NaOH, pH 6.5