5HOC

p73 homo-tetramerization domain mutant II

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-16
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.91741
Spacegroup name	P 42 21 2
Unit cell lengths	60.149, 60.149, 61.564
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.023 - 1.360
R-factor	0.233418540806
Rwork	0.233
R-free	0.24676
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wqi
RMSD bond length	0.010
RMSD bond angle	1.038
Data reduction software	DIALS
Data scaling software	Aimless (0.3.11)
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	60.150	60.150	1.420
High resolution limit [Å]	1.360	4.710	1.360
Rmerge	0.069	0.076	0.465
Rpim	0.021	0.023	0.194
Total number of observations	244067	8714	18278
Number of reflections	24811
<I/σ(I)>	16.3	36.7	2.6
Completeness [%]	99.8	100	99.7
Redundancy	9.8	12.3	6.2
CC(1/2)	0.998	0.994	0.814

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	2 M Sodium chloride, 0.1 M sodium phosphate, 0.1 M potassium phosphate , 0.1 M HEPES pH 7.5