5HNS
Structure of glycosylated NPC1 luminal domain C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-18 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0675 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 87.890, 115.910, 147.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 70.030 - 2.450 |
R-factor | 0.22474 |
Rwork | 0.224 |
R-free | 0.23816 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.519 |
Data reduction software | xia2 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 70.000 |
High resolution limit [Å] | 2.450 |
Number of reflections | 28062 |
<I/σ(I)> | 13.3 |
Completeness [%] | 100.0 |
Redundancy | 26.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | 30% polyethylene glycol mono-ethyl Ether 2000 and 0.1 M potassium thiocyanate |