5HNJ
Base Pairing and Structure Insights into the 5-Formylcytosine in RNA Duplex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-28 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 30.692, 45.923, 45.406 |
Unit cell angles | 90.00, 100.85, 90.00 |
Refinement procedure
Resolution | 25.200 - 1.240 |
R-factor | 0.13454 |
Rwork | 0.134 |
R-free | 0.15451 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 197d |
RMSD bond length | 0.020 |
RMSD bond angle | 2.195 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.280 |
High resolution limit [Å] | 1.240 | 1.240 |
Rmerge | 0.050 | 0.920 |
Number of reflections | 35304 | |
<I/σ(I)> | 35.4 | 2.1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.2 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 50 mM HEPES Na (pH 7.0), 50 mM MgSO4, 1.6 M Li2SO4 |