5HMG

REFINEMENT OF THE INFLUENZA VIRUS HEMAGGLUTININ BY SIMULATED ANNEALING

Experimental procedure

Spacegroup name	P 41
Unit cell lengths	162.600, 162.600, 177.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	? - 3.200
Rwork	0.220
RMSD bond length	0.014
RMSD bond angle	2.800
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	7.000 *
High resolution limit [Å]	3.200 *
Rmerge	0.160 *
Number of reflections	54098 *
Completeness [%]	64.7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	7.5 *		referred to 'Weis, W.', (1988) Nature, 333, 426-431 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	35 (mg/ml)
2	1	2	Na3 citrate	1.30-1.34 (M)
3	1	2		0.1 (%)