5HKC

Crystal structure of M. tuberculosis MazF-mt3 T52D-F62D mutant in complex with 8-mer RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-15
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1
Spacegroup name	P 1 2 1
Unit cell lengths	48.269, 38.798, 69.178
Unit cell angles	90.00, 107.30, 90.00

Refinement procedure

Resolution	33.453 - 1.680
R-factor	0.203
Rwork	0.201
R-free	0.23190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	MazF-mt3
RMSD bond length	0.007
RMSD bond angle	1.179
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.710
High resolution limit [Å]	1.680	4.560	1.680
Rmerge	0.051	0.029	0.458
Rmeas	0.061	0.035	0.570
Rpim	0.034	0.019	0.334
Total number of observations	74834
Number of reflections	27052
<I/σ(I)>	10.9
Completeness [%]	95.9	88.7	98.2
Redundancy	2.8	2.9	2.7
CC(1/2)		0.998	0.723

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	0.2 M Lithium sulfate, 0.1 M Bis-Tris, 25% PEG3350