5HJE
Crystal Structure of Transketolase complex with sedoheptulose-7-phoaphate from Pichia Stipitis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-05-11 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.7 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 100.974, 183.201, 98.435 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.477 - 1.400 |
R-factor | 0.1505 |
Rwork | 0.150 |
R-free | 0.16690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gpu |
RMSD bond length | 0.011 |
RMSD bond angle | 1.186 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX (1.10_2152: ???) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 1.450 |
High resolution limit [Å] | 1.400 | 3.020 | 1.400 |
Rmerge | 0.080 | 0.048 | 0.614 |
Number of reflections | 155291 | ||
<I/σ(I)> | 9.7 | ||
Completeness [%] | 86.8 | 93.4 | 81.1 |
Redundancy | 3.5 | 5 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG400, MES, NaCl |