5HJE

Crystal Structure of Transketolase complex with sedoheptulose-7-phoaphate from Pichia Stipitis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2012-05-11
Detector	RAYONIX MX-300
Wavelength(s)	0.7
Spacegroup name	C 2 2 21
Unit cell lengths	100.974, 183.201, 98.435
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.477 - 1.400
R-factor	0.1505
Rwork	0.150
R-free	0.16690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gpu
RMSD bond length	0.011
RMSD bond angle	1.186
Data scaling software	HKL-2000
Refinement software	PHENIX (1.10_2152: ???)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.450
High resolution limit [Å]	1.400	3.020	1.400
Rmerge	0.080	0.048	0.614
Number of reflections	155291
<I/σ(I)>	9.7
Completeness [%]	86.8	93.4	81.1
Redundancy	3.5	5	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG400, MES, NaCl