5HJD
AF9 YEATS in complex with histone H3 Crotonylation at K18
Experimental procedure
Experimental method | LAUE |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-12-01 |
Detector | MARRESEARCH |
Wavelength(s) | 0.98 - 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 91.264, 101.573, 102.076 |
Unit cell angles | 90.00, 103.06, 90.00 |
Refinement procedure
Resolution | 48.290 - 2.806 |
R-factor | 0.2433 |
Rwork | 0.242 |
R-free | 0.27340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4tmp |
RMSD bond length | 0.004 |
RMSD bond angle | 0.844 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.850 |
High resolution limit [Å] | 2.800 | 7.590 | 2.800 |
Rmerge | 0.116 | 0.062 | 0.880 |
Total number of observations | 321423 | ||
Number of reflections | 49686 | ||
<I/σ(I)> | 9.8 | ||
Completeness [%] | 98.3 | 99.3 | 98.1 |
Redundancy | 3.8 | 3.8 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 277 | 20%(w/v) polyethylene glycol 4000, 0.2 M ammonium sulfate, 0.1 M sodium citrate tribasic dihydrate, 0.1 M copper chloride dihydrate |