5HHM
Crystal Structure of the JM22 TCR in complex with HLA-A*0201 in complex with M1-F5L
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-18 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9537 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 242.876, 47.296, 185.945 |
| Unit cell angles | 90.00, 115.49, 90.00 |
Refinement procedure
| Resolution | 43.580 - 2.500 |
| R-factor | 0.22 |
| Rwork | 0.216 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oga |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.170 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.21) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | BUSTER-TNT (BUSTER 2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.530 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.121 | 0.682 |
| Number of reflections | 67001 | |
| <I/σ(I)> | 7.7 | 2.1 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 50mM Tris-HCl, 70mM NaCl, 16%PEG10K, 14% glycerol |
