5HH1
Crystal structure of human Naa60 mutant - F34A in complex with CoA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-06-28 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97861 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 66.685, 74.031, 43.521 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.521 - 1.803 |
R-factor | 0.1898 |
Rwork | 0.189 |
R-free | 0.20930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5hgz |
RMSD bond length | 0.015 |
RMSD bond angle | 1.581 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.026 | 0.478 |
Number of reflections | 20501 | |
<I/σ(I)> | 27.9 | 2.4 |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 6.3 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIQUID DIFFUSION | 8.5 | 293.15 | 0.2M Lithium Sulfate monohydrate, 0.1M Tris pH 8.5, 20%(w/v) PEG3350 |