5HGU
Crystal structure of human transcription factor TEAD2 in complex with palmitate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9791 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 122.486, 61.146, 80.307 |
| Unit cell angles | 90.00, 117.17, 90.00 |
Refinement procedure
| Resolution | 36.101 - 2.046 |
| R-factor | 0.1866 |
| Rwork | 0.185 |
| R-free | 0.22150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3l15 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.481 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 2.040 |
| Number of reflections | 28795 |
| <I/σ(I)> | 26.3 |
| Completeness [%] | 95.9 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7.2 | 293 | 0.1 M HEPES pH 7.2, 2.4 M sodium formate |
