5HGQ
Loa loa Lysyl-tRNA synthetase in complex with Cladosporin.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 120.165, 147.355, 160.944 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.754 - 3.283 |
| R-factor | 0.2556 |
| Rwork | 0.254 |
| R-free | 0.29230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bju |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.909 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.360 |
| High resolution limit [Å] | 3.280 | 3.300 |
| Rmerge | 0.239 | 0.820 |
| Number of reflections | 40374 | |
| <I/σ(I)> | 4.44 | |
| Completeness [%] | 91.9 | 91.6 |
| Redundancy | 3.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG 3350, magnesium chloride, spermidine |
