5HGJ

Structure of integrin alpha1beta1 and alpha2beta1 I-domains explain differential calcium-mediated ligand recognition

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-04
Detector	PSI PILATUS 6M
Wavelength(s)	0.9791
Spacegroup name	P 1 21 1
Unit cell lengths	37.369, 95.950, 53.078
Unit cell angles	90.00, 104.00, 90.00

Refinement procedure

Resolution	47.975 - 1.399
R-factor	0.1789
Rwork	0.178
R-free	0.20510
RMSD bond length	0.006
RMSD bond angle	1.058
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155))

Data quality characteristics

	Overall
Low resolution limit [Å]	150.000
High resolution limit [Å]	1.360
Number of reflections	67058
<I/σ(I)>	14.7
Completeness [%]	94.6
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	278	PEG 8000, Tris-HCl, Calcium Chloride, Sodium Chloride, Glycerol