5HGC
A Serpin structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-11 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 63 |
| Unit cell lengths | 116.150, 116.150, 72.730 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 58.000 - 2.430 |
| R-factor | 0.16439 |
| Rwork | 0.162 |
| R-free | 0.21692 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hp7 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.968 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.000 | 2.480 |
| High resolution limit [Å] | 2.430 | 2.430 |
| Number of reflections | 21288 | |
| <I/σ(I)> | 11.9 | 1.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.3 | 11.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 200 mM NaCl, 1.75 M Ammonium Sulphate, pH 6.5 |
