5HE0
Bovine GRK2 in complex with Gbetagamma subunits and CCG215022
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-16 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97857 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 60.554, 239.927, 208.872 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.560 |
| R-factor | 0.2048 |
| Rwork | 0.202 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pnk |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.646 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 7.030 | 2.600 |
| Rmerge | 0.067 | 0.023 | 0.391 |
| Rmeas | 0.078 | 0.028 | 0.476 |
| Rpim | 0.040 | 0.015 | 0.268 |
| Total number of observations | 162451 | ||
| Number of reflections | 47002 | ||
| <I/σ(I)> | 12.6 | ||
| Completeness [%] | 97.0 | 95.1 | 86.1 |
| Redundancy | 3.5 | 3.4 | 2.5 |
| CC(1/2) | 0.999 | 0.909 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 50 mM MES, 0.8-1.2 M sodium chloride, 8-16% PEG3350 |
