5HDY
The third PDZ domain from the synaptic protein PSD-95
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-12-13 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97918 |
Spacegroup name | P 41 3 2 |
Unit cell lengths | 89.657, 89.657, 89.657 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.096 - 1.801 |
R-factor | 0.1899 |
Rwork | 0.186 |
R-free | 0.21980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bfe |
RMSD bond length | 0.010 |
RMSD bond angle | 1.161 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10pre_2104) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.096 | 1.866 |
High resolution limit [Å] | 1.801 | 1.801 |
Rmerge | 0.350 | |
Number of reflections | 11131 | |
<I/σ(I)> | 52.621 | 1.313 |
Completeness [%] | 93.2 | |
Redundancy | 9.1 | 8.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 289 | Equal amounts (1.5 microliters) of protein (9 mg/mL) and reservoir solution were mixed and equillibrated against microliters of crystallization buffer (1.0 M sodium citrate) at 289 K. Diamond-shaped crystals appeared either spontaneously or with microseeding after 1-5 days and grew to 100-200 microns in length over several weeks. |