5HDK
Crystal structure of heat shock factor 2-DBD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-03 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97845 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.660, 67.260, 93.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.319 - 1.320 |
| R-factor | 0.1531 |
| Rwork | 0.153 |
| R-free | 0.17440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hdg |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.539 |
| Data reduction software | XSCALE |
| Data scaling software | XDS |
| Phasing software | XDS |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.320 | 1.390 |
| High resolution limit [Å] | 1.320 | 1.320 |
| Rmerge | 0.066 | 0.657 |
| Number of reflections | 95303 | |
| <I/σ(I)> | 3.6 | 3 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 12 | 11.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 293 | 0.1 M Tris, pH 8.4 and 1.25 M potassium sodium tartrate |
