5HCN

GPN-loop GTPase Npa3 in complex with GMPPCP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-01-31
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.99888
Spacegroup name	P 41 21 2
Unit cell lengths	116.240, 116.240, 56.760
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.758 - 2.200
R-factor	0.2139
Rwork	0.214
R-free	0.22700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.145
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.260
High resolution limit [Å]	2.200	2.200
Number of reflections	20172
<I/σ(I)>	28.5	2.07
Completeness [%]	99.5	98
Redundancy	14.11	13.12

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES	7.5	281.15	10 mM HEPES (pH 7.5), 200 mM sodium chloride, 5 mM magnesium chloride, 10 mM DTT