5HCI

GPN-loop GTPase Npa3 in complex with GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-01-31
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.99988
Spacegroup name	C 2 2 21
Unit cell lengths	107.990, 119.180, 347.670
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.248 - 2.300
R-factor	0.2385
Rwork	0.238
R-free	0.27550
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.293
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.360
High resolution limit [Å]	2.300	2.300
Number of reflections	99343
<I/σ(I)>	20.16	1.88
Completeness [%]	99.7	99.8
Redundancy	7.15

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293.15	9 mM HEPES (pH 7), 45 mM sodium chloride, 4.5 mM magnesium chloride, 5% (vol/vol) Jeffamine M-600