5HC1

Structure of EAV NSP11 H141A mutant at 3.10A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-04-20
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1
Spacegroup name	I 2 2 2
Unit cell lengths	129.136, 133.742, 145.268
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 3.100
R-factor	0.21064
Rwork	0.208
R-free	0.25199
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5eyi
RMSD bond length	0.007
RMSD bond angle	1.283
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	BALBES
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.150
High resolution limit [Å]	3.100	3.100
Number of reflections	19651
<I/σ(I)>	21
Completeness [%]	90.7
Redundancy	7.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	PEG