5HA6

Crystal structure of human syncytin-1 fusion subunit

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-14
Detector	RAYONIX MX-300
Wavelength(s)	0.84592
Spacegroup name	H 3 2
Unit cell lengths	48.118, 48.118, 378.911
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.422 - 2.001
R-factor	0.2005
Rwork	0.199
R-free	0.23310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4jf3
RMSD bond length	0.013
RMSD bond angle	1.266
Data reduction software	XDS
Data scaling software	Aimless (0.5.8)
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.100	42.100	2.050
High resolution limit [Å]	2.000	8.950	2.000
Rmerge	0.078	0.030	0.751
Rpim	0.025	0.010	0.257
Total number of observations	126807	1453	7202
Number of reflections	12052
<I/σ(I)>	20.9	54.2	3
Completeness [%]	99.7	99.5	96.1
Redundancy	10.5	8.1	8.8
CC(1/2)	0.999	0.999	0.780

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	298	5% (w/v) PEG 3350, 0.1 M Sodium Acetate-HCl