5HA0
Crystal structure of the LTBP1 leukotriene d4 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-25 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.402, 58.299, 36.039 |
| Unit cell angles | 90.00, 104.14, 90.00 |
Refinement procedure
| Resolution | 33.360 - 1.441 |
| R-factor | 0.1612 |
| Rwork | 0.160 |
| R-free | 0.18820 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 5h9l |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.080 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.460 |
| High resolution limit [Å] | 1.440 | 3.910 | 1.440 |
| Rmerge | 0.067 | 0.042 | 0.247 |
| Rmeas | 0.077 | 0.048 | 0.290 |
| Rpim | 0.036 | 0.023 | 0.148 |
| Total number of observations | 111921 | ||
| Number of reflections | 24723 | ||
| <I/σ(I)> | 9.3 | ||
| Completeness [%] | 99.0 | 98.8 | 96.2 |
| Redundancy | 4.5 | 4.5 | 3.5 |
| CC(1/2) | 0.996 | 0.932 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M Tris pH 8, 30 % PEG 6000 |
