5H8J
Crystal structure of Medicago truncatula N-carbamoylputrescine amidohydrolase (MtCPA) in complex with cadaverine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-08-23 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 152.269, 211.041, 208.822 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.560 - 2.190 |
R-factor | 0.172 |
Rwork | 0.171 |
R-free | 0.21100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1erz |
RMSD bond length | 0.018 |
RMSD bond angle | 1.600 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | BALBES |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.320 |
High resolution limit [Å] | 2.190 | 2.190 |
Rmerge | 0.108 | 0.754 |
Number of reflections | 340107 | |
<I/σ(I)> | 10.1 | 1.93 |
Completeness [%] | 99.1 | 95.3 |
Redundancy | 4.16 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 292 | 20% PEG3350, 8% Tacsimate at pH 7.0, 10% glycerol |