5H38
Structural analysis of KSHV thymidylate synthase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-10-31 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 61.518, 80.545, 65.602 |
Unit cell angles | 90.00, 110.69, 90.00 |
Refinement procedure
Resolution | 19.827 - 1.700 |
R-factor | 0.1682 |
Rwork | 0.167 |
R-free | 0.19460 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hvy |
RMSD bond length | 0.007 |
RMSD bond angle | 1.117 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.730 |
High resolution limit [Å] | 1.695 | 1.700 |
Number of reflections | 65947 | |
<I/σ(I)> | 36.5 | 4.8 |
Completeness [%] | 99.2 | 99.6 |
Redundancy | 4.9 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 300 | potassium phosphate (monobasic), PEG 8K, glycerol |