5H31
Structural basis for dimerization of the death effector domains of Caspase-8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-03-12 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9779 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.774, 51.706, 171.959 |
Unit cell angles | 90.00, 90.05, 90.00 |
Refinement procedure
Resolution | 49.589 - 3.170 |
R-factor | 0.228976096656 |
Rwork | 0.225 |
R-free | 0.26823 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.475 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.150 |
Number of reflections | 15236 |
<I/σ(I)> | 12.2 |
Completeness [%] | 99.7 |
Redundancy | 11.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 8.5 | 293 | 100 mM sodium chloride, 100 mM Tris, 21% PEG3350, 10 mM Sarcosine, |