5H31
Structural basis for dimerization of the death effector domains of Caspase-8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9779 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.774, 51.706, 171.959 |
| Unit cell angles | 90.00, 90.05, 90.00 |
Refinement procedure
| Resolution | 49.589 - 3.170 |
| R-factor | 0.228976096656 |
| Rwork | 0.225 |
| R-free | 0.26823 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.475 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.150 |
| Number of reflections | 15236 |
| <I/σ(I)> | 12.2 |
| Completeness [%] | 99.7 |
| Redundancy | 11.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 8.5 | 293 | 100 mM sodium chloride, 100 mM Tris, 21% PEG3350, 10 mM Sarcosine, |
