5H1Y
Crystal structure of the DDX41 DEAD domain in complex with SO42- and Mg2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 43.881, 62.746, 92.881 |
Unit cell angles | 90.00, 94.32, 90.00 |
Refinement procedure
Resolution | 35.891 - 2.260 |
R-factor | 0.1883 |
Rwork | 0.186 |
R-free | 0.23280 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3fe2 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.234 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.330 |
High resolution limit [Å] | 2.260 | 2.260 |
Number of reflections | 23367 | |
<I/σ(I)> | 21.4 | 3.34 |
Completeness [%] | 98.6 | 97 |
Redundancy | 3.4 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289.15 | 0.2M ammonium sulfate, 25% PEG 3,350, 0.1M Bis-Tris, pH 6.5 |