5H1L
Crystal structure of WD40 repeat domains of Gemin5 in complex with 7-nt U4 snRNA fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.535, 124.790, 61.016 |
| Unit cell angles | 90.00, 117.97, 90.00 |
Refinement procedure
| Resolution | 40.784 - 2.100 |
| R-factor | 0.1971 |
| Rwork | 0.195 |
| R-free | 0.24070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5h1j |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.936 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 4.520 | 2.100 |
| Rmerge | 0.090 | 0.063 | 0.533 |
| Rmeas | 0.108 | ||
| Rpim | 0.059 | ||
| Total number of observations | 153633 | ||
| Number of reflections | 45763 | ||
| <I/σ(I)> | 7.2 | ||
| Completeness [%] | 99.7 | 99.1 | 99.7 |
| Redundancy | 3.4 | 3.3 | 3.4 |
| CC(1/2) | 0.990 | 0.843 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 0.1M Sodium malonate (pH 7.0), 12% PEG 3350 |
