5H1K
Crystal structure of WD40 repeat domains of Gemin5 in complex with 13-nt U4 snRNA fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 98.654, 107.191, 151.480 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| R-factor | 0.17216 |
| Rwork | 0.170 |
| R-free | 0.20626 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5h1j |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.249 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.116 | 0.683 |
| Number of reflections | 126392 | |
| <I/σ(I)> | 17.4 | 2.8 |
| Completeness [%] | 99.7 | 99 |
| Redundancy | 7.9 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | 4% Tacsimate (pH 8.0), 16% PEG 3350 |
