5H0Z
Crystal structure of P113A mutated human transthyretin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-27 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 41.180, 64.260, 85.370 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.130 - 1.735 |
R-factor | 0.2277 |
Rwork | 0.226 |
R-free | 0.25670 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.116 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 32.200 |
High resolution limit [Å] | 1.735 |
Number of reflections | 12061 |
<I/σ(I)> | 7.3 |
Completeness [%] | 99.9 |
Redundancy | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 1.6 M Ammonium sulfate, 0.1 M sodium acetate |