5H0Z
Crystal structure of P113A mutated human transthyretin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-27 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 41.180, 64.260, 85.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.130 - 1.735 |
| R-factor | 0.2277 |
| Rwork | 0.226 |
| R-free | 0.25670 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.116 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 32.200 |
| High resolution limit [Å] | 1.735 |
| Number of reflections | 12061 |
| <I/σ(I)> | 7.3 |
| Completeness [%] | 99.9 |
| Redundancy | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 1.6 M Ammonium sulfate, 0.1 M sodium acetate |
