5H0Y
Crystal structure of H88Y mutated human transthyretin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-06 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 41.349, 62.805, 85.244 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.403 - 1.800 |
R-factor | 0.2055 |
Rwork | 0.203 |
R-free | 0.24330 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.964 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 37.200 |
High resolution limit [Å] | 1.800 |
Number of reflections | 10536 |
<I/σ(I)> | 22.9 |
Completeness [%] | 99.0 |
Redundancy | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 2.0 M ammonium sulfate, 25% glycerol, 0.1 M MES |