5H0X

Crystal structure of H88S mutated human transthyretin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-06
Detector	ADSC QUANTUM 315
Wavelength(s)	1
Spacegroup name	P 21 21 2
Unit cell lengths	42.330, 85.480, 64.060
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.934 - 1.573
R-factor	0.2403
Rwork	0.240
R-free	0.25310
RMSD bond length	0.006
RMSD bond angle	0.851
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX ((1.10.1-2155_1069: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	38.900
High resolution limit [Å]	1.570
Number of reflections	32905
<I/σ(I)>	20.6
Completeness [%]	99.7
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.8	293	1.8 M ammonium sulfate, 25% glycerol, 0.1 M sodium acetate