5GZ3
Structure of D-amino acid dehydrogenase in complex with NADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-12-04 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 63.585, 78.232, 68.969 |
Unit cell angles | 90.00, 107.25, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.590 |
R-factor | 0.1933 |
Rwork | 0.191 |
R-free | 0.22930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5gz1 |
RMSD bond length | 0.030 |
RMSD bond angle | 2.621 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.620 |
High resolution limit [Å] | 1.590 | 4.320 | 1.590 |
Rmerge | 0.084 | 0.046 | 0.458 |
Number of reflections | 81038 | ||
<I/σ(I)> | 16 | ||
Completeness [%] | 93.7 | 78.5 | 83.1 |
Redundancy | 6.3 | 6.1 | 5.6 |
CC(1/2) | 0.996 | 0.840 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | PEG 8000 |