5GY2
Crystal structure of a complex between Bacillus subtilis flagellin and zebrafish Toll-like receptor 5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.146, 54.281, 165.980 |
| Unit cell angles | 90.00, 98.62, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.100 |
| R-factor | 0.19691 |
| Rwork | 0.195 |
| R-free | 0.23627 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3v47 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.507 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.100 |
| Rmerge | 0.091 |
| Number of reflections | 80115 |
| <I/σ(I)> | 16.8 |
| Completeness [%] | 98.1 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.4 | 291 | PEG 300, sodium acetate, pH 4.4 |
