5GWS
4-hydroxyisoleucine dehydrogenase complexed with NADH and succinate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2015-11-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | H 3 |
| Unit cell lengths | 232.709, 232.709, 80.011 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.350 |
| R-factor | 0.21759 |
| Rwork | 0.216 |
| R-free | 0.25355 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3f9i |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.934 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.390 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.093 | |
| Number of reflections | 66938 | |
| <I/σ(I)> | 32.6 | |
| Completeness [%] | 100.0 | |
| Redundancy | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2.0M ammonium sulfate, 0.1M citrate (pH 5.5) |
