5GWB
Hen Egg White Lysozyme native crystals soaked for 2 hours in precipitant solution containing 1 M guanidine hydrochloride and 25% glycerol, before data collection
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-07-20 |
Detector | RIGAKU SATURN A200 |
Wavelength(s) | 1.54187 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 77.360, 77.360, 36.690 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.151 - 2.001 |
R-factor | 0.1841 |
Rwork | 0.182 |
R-free | 0.23310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 193l |
RMSD bond length | 0.008 |
RMSD bond angle | 1.075 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.200 | 2.290 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 7861 | |
<I/σ(I)> | 0.99 | |
Completeness [%] | 99.1 | |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 300 | Sodium Chloride |