5GW9
Crystal structure of C163, a backbone circularized G-CSF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-14 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 32 |
| Unit cell lengths | 60.941, 60.941, 178.509 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.080 - 1.650 |
| R-factor | 0.17939 |
| Rwork | 0.177 |
| R-free | 0.21828 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2d9q |
| RMSD bond length | 0.034 |
| RMSD bond angle | 2.719 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.693 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.062 | 0.640 |
| Number of reflections | 89146 | |
| <I/σ(I)> | 35.5 | 3.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.7 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 293 | 0.4M Ammonium sulfate |
