5GVW
Crystal structure of the apo-form glycosyltransferase GlyE in Streptococcus pneumoniae TIGR4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-04-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97915 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 89.119, 84.211, 130.113 |
Unit cell angles | 90.00, 89.97, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.400 |
R-factor | 0.20106 |
Rwork | 0.198 |
R-free | 0.25465 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5gvv |
RMSD bond length | 0.012 |
RMSD bond angle | 1.541 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.094 | 0.450 |
Number of reflections | 74936 | |
<I/σ(I)> | 11.2 | 2.2 |
Completeness [%] | 99.2 | 98.1 |
Redundancy | 3.1 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.2 M MgCl2, 0.1M HEPES pH 7.5, 25% polyethylene glycol 3350 |