5GVW
Crystal structure of the apo-form glycosyltransferase GlyE in Streptococcus pneumoniae TIGR4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.119, 84.211, 130.113 |
| Unit cell angles | 90.00, 89.97, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.400 |
| R-factor | 0.20106 |
| Rwork | 0.198 |
| R-free | 0.25465 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5gvv |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.541 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.094 | 0.450 |
| Number of reflections | 74936 | |
| <I/σ(I)> | 11.2 | 2.2 |
| Completeness [%] | 99.2 | 98.1 |
| Redundancy | 3.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.2 M MgCl2, 0.1M HEPES pH 7.5, 25% polyethylene glycol 3350 |
