5GVR
Crystal structure of the DDX41 DEAD domain in an apo closed form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-08-19 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.987 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 81.830, 81.830, 69.570 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.010 - 1.500 |
R-factor | 0.18205 |
Rwork | 0.181 |
R-free | 0.20806 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3fe2 |
RMSD bond length | 0.023 |
RMSD bond angle | 2.182 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.010 | 1.510 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.065 | |
Number of reflections | 41380 | |
<I/σ(I)> | 18.4 | |
Completeness [%] | 99.9 | |
Redundancy | 10.34 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 277 | DL-malic acid |