5GV2
Crystal structure of Arginine-bound CASTOR1 from Homo sapiens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.547, 76.834, 95.223 |
| Unit cell angles | 90.00, 96.21, 90.00 |
Refinement procedure
| Resolution | 94.660 - 2.060 |
| R-factor | 0.1925 |
| Rwork | 0.190 |
| R-free | 0.23828 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.871 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 94.700 |
| High resolution limit [Å] | 2.060 |
| Number of reflections | 135965 |
| <I/σ(I)> | 5.7 |
| Completeness [%] | 99.7 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 287 | PEG 3350, Magnesium acetate tetrahydrate |
