5GUV

The crystal structure of mouse DNMT1 (731-1602) mutant - R1279D

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-06
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 41 21 2
Unit cell lengths	79.039, 79.039, 393.276
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	61.602 - 3.078
R-factor	0.2168
Rwork	0.214
R-free	0.26800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3pt9
RMSD bond length	0.013
RMSD bond angle	1.804
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	61.602
High resolution limit [Å]	3.078
Number of reflections	24332
<I/σ(I)>	17.64
Completeness [%]	99.0
Redundancy	27.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	289	25% (w/v) PEG 1500, 100mM SPG, pH 8.5