5GTC
Crystal structure of complex between DMAP-SH conjugated with a Kaposi's sarcoma herpesvirus LANA peptide (5-15) and nucleosome core particle
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-12-15 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.98000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 107.225, 109.017, 176.194 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.590 - 2.700 |
R-factor | 0.214 |
Rwork | 0.211 |
R-free | 0.26690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3afa |
RMSD bond length | 0.011 |
RMSD bond angle | 1.306 |
Data reduction software | Aimless (0.5.17) |
Phasing software | PHASER (2.5.6) |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.590 | 48.590 | 2.760 |
High resolution limit [Å] | 2.690 | 11.710 | 2.690 |
Total number of observations | 57172 | 796 | 3923 |
Number of reflections | 57172 | ||
<I/σ(I)> | 12.3 | 35.7 | 2.2 |
Completeness [%] | 98.3 | 98.6 | 88.4 |
Redundancy | 1 | 1 | 1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | potassium cacodylate, potassium chloride, manganese chloride |