5GT2
Crystal Structure and Biochemical Features of dye-decolorizing peroxidase YfeX from Escherichia coli O157
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 121.574, 101.597, 114.300 |
| Unit cell angles | 90.00, 111.51, 90.00 |
Refinement procedure
| Resolution | 36.693 - 2.093 |
| R-factor | 0.2211 |
| Rwork | 0.220 |
| R-free | 0.24830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.781 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2411: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.090 |
| Number of reflections | 74489 |
| <I/σ(I)> | 16.8 |
| Completeness [%] | 98.0 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M Ammonium sulfate, 20% PEG3350 and 0.1 M Bis-Tris pH 5.5 |
