5GRH
Crystal structure of the alpha gamma heterodimer of human IDH3 in complex with Mg(2+)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-06-02 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 118.397, 118.397, 143.163 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.710 - 2.800 |
R-factor | 0.2257 |
Rwork | 0.224 |
R-free | 0.25920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1t09 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.088 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.710 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 28718 | |
<I/σ(I)> | 9.6 | |
Completeness [%] | 98.9 | |
Redundancy | 10.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M HEPES-Na, pH7.5, 50 mM MgCl2, 30%(v/v) PEGMME 550 |